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pLentiV2-Opti-sgFECH.1-puro
(Plasmid #257585)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257585 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    lentiCRISPRv2-opti (FLAG-removed; Sabatini lab modification)
  • Backbone manufacturer
    Sabatini lab; lentiCRISPRv2-opti, Addgene #163126 (FLAG removed in this Birsoy lab variant)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FECH
  • gRNA/shRNA sequence
    tatatatcttgtggaaaggacgaaacaccgAAGATATGGACTTCCAAGCAgtttaagagctatgctggaaacagcatagc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Entrez Gene
    FECH (a.k.a. EPP, EPP1, FCE)
  • Promoter U6 (sgRNA); EFS (Cas9 + puroR)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (Esp3I) (destroyed during cloning)
  • 3′ cloning site BsmBI (Esp3I) (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

sgRNA construct

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiV2-Opti-sgFECH.1-puro was a gift from Kivanc Birsoy (Addgene plasmid # 257585 ; http://n2t.net/addgene:257585 ; RRID:Addgene_257585)

  • For your References section:

    Autoregulatory control of mitochondrial glutathione homeostasis. Liu Y, Liu S, Tomar A, Yen FS, Unlu G, Ropek N, Weber RA, Wang Y, Khan A, Gad M, Peng J, Terzi E, Alwaseem H, Pagano AE, Heissel S, Molina H, Allwein B, Kenny TC, Possemato RL, Zhao L, Hite RK, Vinogradova EV, Mansy SS, Birsoy K. Science. 2023 Nov 17;382(6672):820-828. doi: 10.1126/science.adf4154. Epub 2023 Nov 2. 10.1126/science.adf4154 PubMed 37917749
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