EGFP-PhosTAP_PP1
(Plasmid
#257702)
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Purposeexpresses EGFP linked PhosTAP_PP1 for the inhibition of Protein Phosphatase 1 binding (SLiM) sites
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257702 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP_PhosTap_PP1
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Alt namePhosTap_PP1
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SpeciesSynthetic
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-PhosTAP_PP1 was a gift from Wolfgang Peti (Addgene plasmid # 257702 ; http://n2t.net/addgene:257702 ; RRID:Addgene_257702) -
For your References section:
A protein phosphatase 1 specific phosphatase targeting peptide (PhosTAP) to identify the PP1 phosphatome. Choy MS, Nguyen HT, Kumar GS, Peti W, Kettenbach AN, Page R. Proc Natl Acad Sci U S A. 2024 Oct 29;121(44):e2415383121. doi: 10.1073/pnas.2415383121. Epub 2024 Oct 24. 10.1073/pnas.2415383121 PubMed 39446389