Donor plasmid_lacZa insert
(Plasmid
#257707)
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PurposeDonor plasmid for T4 phage genome editing with homologous arms and lacZα insert
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Depositing Lab
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Depositing OrganizationUniversity of California, Riverside
Located in the United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepZE21-MCS1
- Backbone size w/o insert (bp) 2254
- Total vector size (bp) 4936
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelacZα
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SpeciesEscherichia coli
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Insert Size (bp)480
Resource Information
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A portion of this plasmid was derived from a plasmid made byDerived from Addgene plasmid #42875 and modified by inserting T4 phage homologous arms and the lacZα cassette for phage genome editing.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Donor plasmid_lacZa insert was a gift from Juhong Chen (Addgene plasmid # 257707 ; http://n2t.net/addgene:257707 ; RRID:Addgene_257707) -
For your References section:
CRISPR/Cas9-Mediated Genome Editing of T4 Bacteriophage for High-Throughput Antimicrobial Susceptibility Testing. He Y, Chen J. Anal Chem. 2024 Nov 12;96(45):18301-18310. doi: 10.1021/acs.analchem.4c05177. Epub 2024 Oct 30. 10.1021/acs.analchem.4c05177 PubMed 39474820
