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pRSFDuet_PflACD
(Plasmid #257730)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257730 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSFDuet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PflA, PflC, PflD
  • Species
    Pseudomonas fluorescens CH267
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.30.685615 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDuet_PflACD was a gift from Wilfred van der Donk (Addgene plasmid # 257730 ; http://n2t.net/addgene:257730 ; RRID:Addgene_257730)

  • For your References section:

    Oxidative Peptide Backbone Cleavage by a HEXXH Enzyme during RiPP Biosynthesis. Ouyang Y, Yu Y, Zhu L, Nguyen DT, van der Donk WA. J Am Chem Soc. 2026 Jan 28;148(3):3551-3561. doi: 10.1021/jacs.5c19246. Epub 2026 Jan 18. 10.1021/jacs.5c19246 PubMed 41549476
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