Oxidative Peptide Backbone Cleavage by a HEXXH Enzyme during RiPP Biosynthesis.
Ouyang Y, Yu Y, Zhu L, Nguyen DT, van der Donk WA
J Am Chem Soc. 2026 Jan 28;148(3):3551-3561. doi: 10.1021/jacs.5c19246. Epub 2026 Jan 18.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 257729 | pRSFDuet_PflABD | Coexpression of DUF692 protein PflD, GNAT PflB, and the precursor peptide PflA |
| 257730 | pRSFDuet_PflACD | Coexpression of DUF692 protein PflD, HEXXH protein PflC, and the precursor peptide PflA |
| 257731 | pRSFDuet_PosABCD | Coexpression of DUF692 protein PosD, the HEXXH alphaKG-dependent oxidase PosC, and the precursor peptide PosA. PosB and PosD are inactive. |