Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25774||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)3000
Entrez Genepcdh8 (a.k.a. papc, zPAPC)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
antisense: linearize with Apa1, transcribe with T3
sense: linearize with Not1, transcribe with T7
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS Zpapc was a gift from Edward De Robertis (Addgene plasmid # 25774 ; http://n2t.net/addgene:25774 ; RRID:Addgene_25774)
For your References section:Zebrafish paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm. Yamamoto A, Amacher SL, Kim SH, Geissert D, Kimmel CB, De Robertis EM. Development. 1998 Sep . 125(17):3389-97. PubMed 9693142
Map uploaded by the depositor.