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pETDuet-His-SUMO-NpuAC20A
(Plasmid #257741)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257741 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pETDuet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NpuA
  • Species
    Nostoc punctiforme PCC 73102
  • Mutation
    C20A
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.01.31.703028 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-His-SUMO-NpuAC20A was a gift from Wilfred van der Donk (Addgene plasmid # 257741 ; http://n2t.net/addgene:257741 ; RRID:Addgene_257741)

  • For your References section:

    Structure, Biosynthesis, and Bioactivity of Nostolysamides. Weir E, Anterola I, van der Donk WA. Biochemistry. 2026 May 5;65(9):1578-1591. doi: 10.1021/acs.biochem.6c00103. Epub 2026 Apr 24. 10.1021/acs.biochem.6c00103 PubMed 42032808
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