Structure, Biosynthesis, and Bioactivity of Nostolysamides.
Weir E, Anterola I, van der Donk WA
Biochemistry. 2026 May 5;65(9):1578-1591. doi: 10.1021/acs.biochem.6c00103. Epub 2026 Apr 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 257732 | pRSFDuet_His-SUMO-NpuN | Expression of NpuN, the acetyltransferase protein, for in vitro reaction |
| 257733 | pRSFduet_His-NpuM | Expression of NpuM, the lanthionine synthase, and for co-expression with the precursor peptide and NpuA mutants |
| 257735 | pETDuet-His-SUMO-NpuA-NpuM | For expression to understand PTM and characterized |
| 257736 | pETDuet-His-SUMO-TEV-NpuAK1A | For expression and co-expression with the modifying enzyme in the pathway, NpuN (acetyltransferase) |
| 257737 | pETDuet-His-SUMO-TEV-NpuAK3A | For expression and co-expression with the modifying enzyme in the pathway, NpuN (acetyltransferase) |
| 257738 | pETDuet-His-SUMO-TEV-NpuAK1AK3A | For expression and co-expression with the modifying enzyme in the pathway, NpuN (acetyltransferase) |
| 257739 | pETDuet-His-SUMO-NpuAS4A-NpuM | For expression to understand where dehydrations occurred |
| 257740 | pETDuet-His-SUMO-NpuAC14A | For expression and co-expression with the modifying enzyme in the pathway, NpuM |
| 257741 | pETDuet-His-SUMO-NpuAC20A | For expression and co-expression with the modifying enzyme in the pathway, NpuM |
| 257742 | pETDuet-His-SUMO-NpuAC25A | For expression and co-expression with the modifying enzyme in the pathway, NpuM |