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pET28a-msrB
(Plasmid #257815)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257815 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Methionine sulfoxide reductase B
  • Alt name
    MsrB
  • Species
    E. coli
  • Insert Size (bp)
    414
  • GenBank ID
    LR883050.1
  • Tag / Fusion Protein
    • His6-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://chemrxiv.org/doi/10.26434/chemrxiv-2025-nnx2v for ChemRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-msrB was a gift from Seraphine Wegner (Addgene plasmid # 257815 ; http://n2t.net/addgene:257815 ; RRID:Addgene_257815)

  • For your References section:

    Restoring intracellular homeostasis disrupted by synthetic nanoassemblies. Xing J, Zheng X, Ren Y, Schuler M, Ng DYW, Weil T, Wegner SV, Synatschke CV. Nat Chem Biol. 2026 Oct;22(10):1676-1684. doi: 10.1038/s41589-026-02279-x. Epub 2026 Jul 17. 10.1038/s41589-026-02279-x PubMed 42469449
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