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pL1L2 rtTA-FLAG (AJ13)
(Plasmid #257874)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257874 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pL1L2 (pME with modified MCS)
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Gateway Cloning ; Tet-On

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-tagged reverse Tet-On transactivator rtTA
  • Species
    Synthetic
  • Promoter none
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Invitrogen

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pL1L2 rtTA-FLAG (AJ13) was a gift from Abigail Jensen (Addgene plasmid # 257874 ; http://n2t.net/addgene:257874 ; RRID:Addgene_257874)

  • For your References section:

    Two types of Tet-On transgenic lines for doxycycline-inducible gene expression in zebrafish rod photoreceptors and a gateway-based tet-on toolkit. Campbell LJ, Willoughby JJ, Jensen AM. PLoS One. 2012;7(12):e51270. doi: 10.1371/journal.pone.0051270. Epub 2012 Dec 12. 10.1371/journal.pone.0051270 PubMed 23251476
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