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Two types of Tet-On transgenic lines for doxycycline-inducible gene expression in zebrafish rod photoreceptors and a gateway-based tet-on toolkit.

Campbell LJ, Willoughby JJ, Jensen AM
PLoS One. 2012;7(12):e51270. doi: 10.1371/journal.pone.0051270. Epub 2012 Dec 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
257872p5E_TRE (AJ4) 5' Gateway plasmid with tet-On TRE for use in building transgenics.
257873pL1L2 rtTA-HA (AJ12) Middle entry Gateway plasmid with HA-tagged Tet-On transactivator gene to make conditional transgenics
257874 pL1L2 rtTA-FLAG (AJ13)Middle entry Gateway plasmid with FLAG-tagged Tet-On transactivator gene to make conditional transgenics
257877p5E-dA_MCS-bi-TRE (AJ22) 5' Gateway plasmid with SV40 dA_MCS_biTRE' cassette for use in building transgenics.

Antibodies from Article