psPAX2_D64V_NC_mScarlet
(Plasmid
#257942)
-
PurposeFor integrase-deficient, mScarlet-labeled virus-like particle production in mammalian cells
-
Depositing Lab
-
Depositing OrganizationRockefeller University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257942 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepsPAX2
- Total vector size (bp) 11452
-
Vector typeMammalian Expression, Lentiviral ; Packaging
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namegag pol
-
Tag
/ Fusion Protein
- mScarlet
Resource Information
-
A portion of this plasmid was derived from a plasmid made byHoward Chang (Addgene Plasmid #196509)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.05.14.725198 for bioRxiv preprint.
Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. The depositor confirms this change is not expected to impact plasmid function. Please refer to the Addgene sequence for the most accurate result.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
psPAX2_D64V_NC_mScarlet was a gift from Jean-Laurent Casanova (Addgene plasmid # 257942 ; http://n2t.net/addgene:257942 ; RRID:Addgene_257942) -
For your References section:
A Flow Cytometry-Based Assay to Quantify the Binding of Transmembrane Ligands to Their Cognate Receptors Using Fluorescent Virus-Like Particles. Kim CM, Gaballa M, Lee D, Jouanguy E, Zhang SY, Casanova JL, Yatim A. Bio Protoc. 2026 Jul 5;16(13):e5726. doi: 10.21769/BioProtoc.5726. eCollection 2026 Jul 5. 10.21769/BioProtoc.5726 PubMed 42427450