A Flow Cytometry-Based Assay to Quantify the Binding of Transmembrane Ligands to Their Cognate Receptors Using Fluorescent Virus-Like Particles.
Kim CM, Gaballa M, Lee D, Jouanguy E, Zhang SY, Casanova JL, Yatim A
Bio Protoc. 2026 Jul 5;16(13):e5726. doi: 10.21769/BioProtoc.5726. eCollection 2026 Jul 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 257941 | psPAX2_D64V_NC_TagBFP | For integrase-deficient, TagBFP-labeled virus-like particle production in mammalian cells |
| 257960 | pCMV6_ICAM-1 WT | Expresses wild-type ICAM-1 in mammalian cells |
| 257961 | pCMV6_ICAM-1 E34A | Expresses ICAM-1 containing the E34A mutation, abrogating binding to LFA-1, in mammalian cells |