pCMV6_ICAM-1 E34A
(Plasmid
#257961)
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PurposeExpresses ICAM-1 containing the E34A mutation, abrogating binding to LFA-1, in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 257961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCMV6
- Total vector size (bp) 6375
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameICAM-1 (CD54)
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SpeciesH. sapiens (human)
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MutationE34A
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Entrez GeneICAM1 (a.k.a. BB2, CD54, P3.58)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.05.14.725198 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV6_ICAM-1 E34A was a gift from Jean-Laurent Casanova (Addgene plasmid # 257961 ; http://n2t.net/addgene:257961 ; RRID:Addgene_257961) -
For your References section:
A Flow Cytometry-Based Assay to Quantify the Binding of Transmembrane Ligands to Their Cognate Receptors Using Fluorescent Virus-Like Particles. Kim CM, Gaballa M, Lee D, Jouanguy E, Zhang SY, Casanova JL, Yatim A. Bio Protoc. 2026 Jul 5;16(13):e5726. doi: 10.21769/BioProtoc.5726. eCollection 2026 Jul 5. 10.21769/BioProtoc.5726 PubMed 42427450