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pCMV6_ICAM-1 WT
(Plasmid #257960)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257960 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCMV6
  • Total vector size (bp) 6375
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ICAM-1 (CD54)
  • Species
    H. sapiens (human)
  • Entrez Gene
    ICAM1 (a.k.a. BB2, CD54, P3.58)
  • Promoter CMV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Timothy Springer (Addgene Plasmid #8632)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.05.14.725198 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6_ICAM-1 WT was a gift from Jean-Laurent Casanova (Addgene plasmid # 257960 ; http://n2t.net/addgene:257960 ; RRID:Addgene_257960)

  • For your References section:

    A Flow Cytometry-Based Assay to Quantify the Binding of Transmembrane Ligands to Their Cognate Receptors Using Fluorescent Virus-Like Particles. Kim CM, Gaballa M, Lee D, Jouanguy E, Zhang SY, Casanova JL, Yatim A. Bio Protoc. 2026 Jul 5;16(13):e5726. doi: 10.21769/BioProtoc.5726. eCollection 2026 Jul 5. 10.21769/BioProtoc.5726 PubMed 42427450
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