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pMSP1E3D1_HaloTag
(Plasmid #257990)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257990 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone manufacturer
    GenScript
  • Backbone size w/o insert (bp) 1548
  • Total vector size (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MSP1E3D1_HaloTag
  • Species
    Synthetic
  • Promoter T7 Promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco1 (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Stephen Sligar (https://www.addgene.org/20066/). The HaloTag protein sequence was derived from DOI: https://doi.org/10.1038/s41592-021-01341-x.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSP1E3D1_HaloTag was a gift from Michael Marty (Addgene plasmid # 257990 ; http://n2t.net/addgene:257990 ; RRID:Addgene_257990)
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