Design of Fluorescent Membrane Scaffold Proteins for Nanodiscs

Cleveland E, Wolf AR, Chen S, Mohona FA, Kailat I, Tran BH, Lavanya SB; Primanis-Erickson N, Lin Y-C, Marty MT
ACS Chem Biol. (2026). doi: https://doi.org/10.1021/acschembio.6c00340 Article

Plasmids from Article

ID	Plasmid	Purpose
248920	pMSP1D1_sfGFP	Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to sfGFP for nanodisc formation
248922	pMSP1D1_mCherry	Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to mCherry for nanodisc formation
248923	pMSP1D1_sfCherry	Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to sfCherry for nanodisc formation
248924	pMSP1D1_sfCherryL	Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to sfCherry for nanodisc formation (longer linker)
248925	pMSP1D1_mBlueberry2	Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to mBlueberry2 for nanodisc formation
248926	pMSP1D1_HaloTag	Bacterial expression of Halo-tagged membrane scaffold protein MSP1D1 fused to HaloTag for nanodisc formation
248927	pMSP1E3D1_sfGFP	Bacterial expression of fluorescent membrane scaffold protein MSP1E3D1 fused to sfGFP for nanodisc formation
248929	pMSP1E3D1_mCherry	Bacterial expression of fluorescent membrane scaffold protein MSP1E3D1 fused to mCherry for nanodisc formation
248930	pMSP1E3D1_mBlueberry2	Bacterial expression of fluorescent membrane scaffold protein MSP1E3D1 fused to mBlueberry 2 for nanodisc formation
257989	pMSP1E3D1_sfCherry3C	Bacterial expression of fluorescent membrane scaffold protein for nanodisc formation
257990	pMSP1E3D1_HaloTag	Bacterial expression of fluorescent membrane scaffold protein for nanodisc formation
257991	pMSP1D1_mCherryXL	Bacterial expression of fluorescent membrane scaffold protein for nanodisc formation
257992	Annexin V-sfGFP	Bacterial expression of fluorescent Annexin V