SBC016302
(Plasmid
#258001)
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PurposeExpression of Glycine max 4CL4, Arabidopsis thaliana CHS and CHI2, together with an FdeR‑based biosensor, for the production and detection of flavanones.
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Depositing Lab
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Depositing OrganizationUniversity of Manchester
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBbE5k
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert name4-Coumarate-CoA ligase
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Alt name4CL4
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SpeciesGlycine max
Gene/Insert 2
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Gene/Insert nameChalcone synthase
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Alt nameCHS
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SpeciesA. thaliana (mustard weed)
Gene/Insert 3
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Gene/Insert nameChalcone isomerase
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Alt nameCHI2
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SpeciesA. thaliana (mustard weed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SBC016302 was a gift from Nigel Scrutton (Addgene plasmid # 258001 ; http://n2t.net/addgene:258001 ; RRID:Addgene_258001) -
For your References section:
Biosensor-guided evolution of chalcone synthase enhances biosynthesis of natural and non-natural flavanones. Hanko EKR, Kesornpun C, Whitehead JN, Spiess R, Robinson CJ, Scrutton NS. Metab Eng. 2026 Oct 7:102564. doi: 10.1016/j.ymben.2026.102564. 10.1016/j.ymben.2026.102564 PubMed 42843616