SBC016420
(Plasmid #258003)

• Purpose: Expression of Glycine max 4CL1, Hordeum vulgare CHS2, and Arabidopsis thaliana CHI2, together with an FdeR‑based biosensor, for the production and detection of flavanones.
• Depositing Lab: Nigel Scrutton
• Depositing Organization: University of Manchester
  Located in the United Kingdom of Great Britain and Northern Ireland
• Publication: Hanko et al Metab Eng. 2026 Oct 7:102564. doi: 10.1016/j.ymben.2026.102564.

Backbone

• Vector backbone: pBbE5k
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: 4-Coumarate-CoA ligase
• Alt name: 4CL1
• Species: Glycine max

Gene/Insert 2

• Gene/Insert name: Chalcone synthase
• Alt name: CHS2
• Species: Hordeum vulgare

Gene/Insert 3

• Gene/Insert name: Chalcone isomerase
• Alt name: CHI2
• Species: A. thaliana (mustard weed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry