pLV-puro-NUDT5-3xFLAG
(Plasmid
#258079)
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PurposeOverexpression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258079 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLV-EF1a-IRES-puro
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Backbone manufacturerTobias Meyer, Addgene plasmid #85132
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNUDT5
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Alt nameNUDIX5
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SpeciesH. sapiens (human)
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Insert Size (bp)750
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Entrez GeneNUDT5 (a.k.a. YSA1, YSA1H, YSAH1, hNUDT5)
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer EF1alpha
- 3′ sequencing primer IRES-rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-puro-NUDT5-3xFLAG was a gift from Alexis Jourdain (Addgene plasmid # 258079 ; http://n2t.net/addgene:258079 ; RRID:Addgene_258079)