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pFAST-His-TEV-mEGFP-TEV-FAK-W266A
(Plasmid #258176)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258176 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pFastBacHTa2
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ptk2
  • Alt name
    FAK
  • Species
    M. musculus (mouse)
  • Mutation
    Tryptophan 266 is mutated to alanine (W266A) which disrupts FERM domain mediated dimerization.
  • Entrez Gene
    Ptk2 (a.k.a. FADK 1, FAK, FRNK, Fadk, p125FAK)
  • Promoter polh
  • Tag / Fusion Protein
    • his(6)tev (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFAST-His-TEV-mEGFP-TEV-FAK-W266A was a gift from Lindsay Case (Addgene plasmid # 258176 ; http://n2t.net/addgene:258176 ; RRID:Addgene_258176)
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