Lindsay Case Lab: Kinase condensates enrich ATP and trigger autophosphorylation

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
258174	XLone-Puro-mEGFP-FAK-WT	Used to generate a stable, Dox-inducible mEGFP-FAK mamallian cell line when coexpressed with the PiggyBac transposase
258175	pFAST-His-TEV-mEGFP-TEV-FAK-WT	Used for generation of bacmid DNA and subsequent baculovirus for insect cell expression/purification of FAK.
258176	pFAST-His-TEV-mEGFP-TEV-FAK-W266A	Used for generation of bacmid DNA and subsequent baculovirus for insect cell expression/purification of FAK-W266A.
258178	pFAST-His-mEGFP-TEV-Abl-WT	Used for generation of bacmid DNA and subsequent baculovirus for insect cell expression/purification of Abl-WT.
258179	pFAST-His-mEGFP-TEV-Abl-RS	Used for generation of bacmid DNA and subsequent baculovirus for insect cell expression/purification of Abl-RS.
258180	pCIOX-Mst2	Used for expression of Mst2 with an N-terminal His-SUMO tag in E. coli for purification.
258181	pCIOX-mEGFP-Sav1-MBP	Used for expression of SAV1 with an N-terminal mEGFP and C-terminal MBP tag in E. coli for purification.
258182	pCIOX-FAK-IDR	Used for expression of the FAK IDR with an N-terminal His-SUMO tag in E. coli for purification.
258183	pCIOX-Abl-IDR	Used for expression of the Abl IDR with an N-terminal His-SUMO tag in E. coli for purification.
258184	pCIOX-DDX21-C-IDR-WT	Used for expression of the DDX21 C-terminal IDR with an N-terminal His-SUMO tag in E. coli for purification.
258185	pCIOX-DDX21-C-IDR-RS	Used for expression of the DDX21 C-terminal IDR (RS) with an N-terminal His-SUMO tag in E. coli for purification.
258186	pGEX-PTP1B	Used for expression of PTP1B with an N-terminal GST tag in E. coli for purification.