pCIOX-DDX21-C-IDR-WT
(Plasmid
#258184)
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PurposeUsed for expression of the DDX21 C-terminal IDR with an N-terminal His-SUMO tag in E. coli for purification.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258184 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCIOX
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDDX21
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SpeciesH. sapiens (human)
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Entrez GeneDDX21 (a.k.a. GUA, GURDB, II/Gu, RH, RH II/Gu, RH-II/GU, RH-II/GuA, gu-alpha)
- Promoter T7
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Tag
/ Fusion Protein
- his(8)sumo (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Encodes residues 716-783
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIOX-DDX21-C-IDR-WT was a gift from Lindsay Case (Addgene plasmid # 258184 ; http://n2t.net/addgene:258184 ; RRID:Addgene_258184)