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Sc RFC[hk-1s + 5(SAC)]-pLANT/RIL
(Plasmid #258209)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258209 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLANT(3b)/RIL
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    RFC1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    N-terminally truncated
  • Entrez Gene
    RFC1 (a.k.a. YOR217W, CDC44)
  • Tag / Fusion Protein
    • His(10) followed by kinase (PKA) site (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Gene/Insert 2

  • Gene/Insert name
    RFC5
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    RFC5 (K184A) (SAC)
  • Entrez Gene
    RFC5 (a.k.a. YBR087W)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mix & Match (co-transform) with different versions of Sc RFC[2+3+4], either wild-type or containing various mutations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sc RFC[hk-1s + 5(SAC)]-pLANT/RIL was a gift from Michael O'Donnell (Addgene plasmid # 258209 ; http://n2t.net/addgene:258209 ; RRID:Addgene_258209)
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