Michael O'Donnell Lab: O'Donnell Lab Plasmids May 2026
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 258189 | Sc RFC[2 + 3 + 4(SAC)]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with RFC4 containing a zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258190 | Sc RFC[2 + 3 + 4(GAT)]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with RFC4 containing a P-loop (GAT) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258191 | Sc RFC[2 + 3 + 4(GAT/SAC)]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with RFC4 containing both a P-loop (GAT) and zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258192 | Sc RFC[2 + 3(SAC) + 4]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with RFC3 containing a zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258193 | Sc RFC[2 + 3(SAC) + 4(SAC)]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with both RFC3 & RFC4 containing a zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258194 | Sc RFC[2 + 3(GAT) + 4]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with RFC3 containing a P-loop (GAT) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258195 | Sc RFC[2 + 3(GAT) + 4(GAT)]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with both RFC3 & RFC4 containing a P-loop (GAT) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258196 | Sc RFC[2 + 3(GAT) + 4(GAT/SAC)]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4; RFC3 has P-loop (GAT) mutation and RFC4 has both P-loop (GAT) & zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258197 | Sc RFC[2 + 3(GAT/SAC) + 4]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with RFC3 containing both a P-loop (GAT) and zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258198 | Sc RFC[2 + 3(GAT/SAC) + 4(GAT)]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4; RFC3 has both P-loop (GAT) & zinc-finger (SAC) mutation and RFC4 has P-loop (GAT) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258199 | Sc RFC[2(SAC) + 3 + 4]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with RFC2 containing a zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258200 | Sc RFC[2(SAC) + 3 + 4(SAC)]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with both RFC2 & RFC4 containing a zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258201 | Sc RFC[2(SAC) + 3(SAC) + 4]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with both RFC2 & RFC3 containing a zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258202 | Sc RFC[2(SAC) + 3(SAC) + 4(SAC)]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with all three genes (RFC2, RFC3, & RFC4) containing a zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258203 | Sc RFC[2(SAC) + 3(GAT) + 4]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, where RFC2 has a zinc-finger (SAC) mutation and RFC3 has a P-loop (GAT) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258204 | Sc RFC[2(GAT) + 3 + 4]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with RFC2 containing a P-loop (GAT) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258206 | Sc RFC[2(GAT) + 3(SAC) + 4]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, where RFC2 has a P-loop (GAT) mutation and RFC3 has a zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258207 | Sc RFC[2(GAT) + 3(SAC) + 4(SAC)]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, where RFC2 has P-loop (GAT) mutation and both RFC3 & RFC4 have zinc-finger (SAC) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258208 | Sc RFC[2(GAT) + 3(GAT) + 4]-pET | Over-express in E. coli Sc RFC2, RFC3, & RFC4, with both RFC2 & RFC3 containing a P-loop (GAT) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1. |
| 258212 | Ec his-FLAG-dinB-pET(16b) (Pol IV) | Over-express in E. coli Ec dinB (Pol IV) with N-terminal His(10) & 3X FLAG tags. |