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pGEX6P-GST-XlH1M
(Plasmid #258230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258230 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRUTH5 GST
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H1.M
  • Alt name
    Histone H1M
  • Alt name
    B4
  • Species
    X. laevis (frog)
  • Tags / Fusion Proteins
    • 6xHis-TEV (N terminal on insert)
    • Strep-tag II (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6P-GST-XlH1M was a gift from David Shechter (Addgene plasmid # 258230 ; http://n2t.net/addgene:258230 ; RRID:Addgene_258230)

  • For your References section:

    Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiency. Lorton BM, Warren C, Ilyas H, Nandigrami P, Hegde S, Cahill S, Lehman SM, Shabanowitz J, Hunt DF, Fiser A, Cowburn D, Shechter D. iScience. 2024 Mar 8;27(4):109458. doi: 10.1016/j.isci.2024.109458. eCollection 2024 Apr 19. 10.1016/j.isci.2024.109458 PubMed 38571760
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