Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiency.
Lorton BM, Warren C, Ilyas H, Nandigrami P, Hegde S, Cahill S, Lehman SM, Shabanowitz J, Hunt DF, Fiser A, Cowburn D, Shechter D
iScience. 2024 Mar 8;27(4):109458. doi: 10.1016/j.isci.2024.109458. eCollection 2024 Apr 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 258226 | pRUTH5_XlH2A | E. coli expression of Xenopus laevis H2A with an N-terminal cleavable His tag |
| 258227 | pRUTH5_XlH2B | E. coli expression of Xenopus laevis H2B with an N-terminal cleavable His tag |
| 258228 | pRUTH5_XlH3 | E. coli expression of Xenopus laevis H3.2 with an N-terminal cleavable His tag |
| 258229 | pRUTH5-GST-XlH1A | E. coli expression of Xenopus laevis H1A with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II |
| 258230 | pGEX6P-GST-XlH1M | E. coli expression of Xenopus laevis H1M with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II |
| 258231 | pRUTH5_XlH2A-StrepII | E. coli expression of Xenopus laevis H2A with an N-terminal cleavable His tag and C-terminal StrepII tag and a C-terminal Strep-tag II |
| 258233 | pRUTH5_Xl-NPM2 | E. coli expression of Xenopus laevis full length NPM2 with an N-terminal cleavable His tag |
| 258234 | pRUTH5_Xl-NPM2-Core | E. coli expression of Xenopus laevis NPM2 core with an N-terminal cleavable His tag |
| 258235 | pRUTH5_Xl-NPM2-Core+A2 | E. coli expression of Xenopus laevis NPM2 core + A2 with an N-terminal cleavable His tag |
| 258236 | pRUTH5-GST_Xl-NPM2-Cterm | E. coli expression of Xenopus laevis NPM2 Cterm (past A3) with an N-terminal cleavable His-GST tag |
| 258239 | pRUTH5_Xl-Nap1 delta A3 | E. coli expression of Xenopus laevis Nap1L1.L delta A3 with an N-terminal cleavable His tag |
| 258240 | pRUTH5_Xl-Nap1 core | E. coli expression of Xenopus laevis Nap1L1.L core domain with an N-terminal cleavable His tag |