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Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiency.

Lorton BM, Warren C, Ilyas H, Nandigrami P, Hegde S, Cahill S, Lehman SM, Shabanowitz J, Hunt DF, Fiser A, Cowburn D, Shechter D
iScience. 2024 Mar 8;27(4):109458. doi: 10.1016/j.isci.2024.109458. eCollection 2024 Apr 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
258230pGEX6P-GST-XlH1ME. coli expression of Xenopus laevis H1M with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II
258231pRUTH5_XlH2A-StrepIIE. coli expression of Xenopus laevis H2A with an N-terminal cleavable His tag and C-terminal StrepII tag and a C-terminal Strep-tag II

Antibodies from Article