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pECFB1_HsMEP50
(Plasmid #258245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258245 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pECFB-1
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WDR77
  • Alt name
    MEP50
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1023
  • Entrez Gene
    WDR77 (a.k.a. HKMT1069, MEP-50, MEP50, Nbla10071, p44, p44/Mep50)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECFB1_HsMEP50 was a gift from David Shechter (Addgene plasmid # 258245 ; http://n2t.net/addgene:258245 ; RRID:Addgene_258245)

  • For your References section:

    Productive mRNA chromatin escape is promoted by PRMT5 activity. DeAngelo JD, Maron MI, Roth JS, Hegde S, Silverstein AM, Gupta V, Stransky S, Basken J, Azofeifa J, Query CC, Sidoli S, Gamble MJ, Shechter D. Mol Cell. 2025 Nov 6;85(21):4016-4031.e9. doi: 10.1016/j.molcel.2025.09.021. Epub 2025 Oct 13. 10.1016/j.molcel.2025.09.021 PubMed 41086806
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