Productive mRNA chromatin escape is promoted by PRMT5 activity.
DeAngelo JD, Maron MI, Roth JS, Hegde S, Silverstein AM, Gupta V, Stransky S, Basken J, Azofeifa J, Query CC, Sidoli S, Gamble MJ, Shechter D
Mol Cell. 2025 Nov 6;85(21):4016-4031.e9. doi: 10.1016/j.molcel.2025.09.021. Epub 2025 Oct 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 258244 | pECFB1_HsPRMT5 | Baculovirus/insect cell expression of human PRMT5 with an N-terminal cleavable His tag |
| 258245 | pECFB1_HsMEP50 | Baculovirus/insect cell expression of human MEP50/WDR77 with an N-terminal cleavable His tag |
| 258246 | pXPR_050_PRMT5-3 | Lentiviral expression of CRISPRi PRMT5 sgRNA #3 |
| 258247 | pXPR_050_PRMT5-4 | Lentiviral expression of CRISPRi PRMT5 sgRNA #4 |
| 258248 | pXPR_050_CoPR5-1 | Lentiviral expression of CRISPRi CoPR5 sgRNA #1 |
| 258249 | pXPR_050_CoPR5-3 | Lentiviral expression of CRISPRi CoPR5 sgRNA #3 |
| 258250 | pXPR_050_pICln-2 | Lentiviral expression of CRISPRi pICln sgRNA #2 |
| 258251 | pXPR_050_pICln-3 | Lentiviral expression of CRISPRi pICln sgRNA #3 |
| 258252 | pXPR_050_RIOK1-1 | Lentiviral expression of CRISPRi RIOK1 sgRNA #1 |
| 258253 | pXPR_050_RIOK1-3 | Lentiviral expression of CRISPRi RIOK1 sgRNA #3 |
| 258254 | pLIX403_HA-SNRPB_WT | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPB |
| 258256 | pLIX403_HA-SNRPB_RtoA | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPB with arginine-to-alanine mutations |
| 258257 | pLIX403_HA-SNRPB_RtoAx2 | Lentiviral inducible expression of N-terminal HA-tagged human SNRPB with two arginine-to-alanine mutations |
| 258258 | pRUTH5-GST_SNRPB-StrepII | E. coli expression of human SNRPB C-terminal domain with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II |
| 258259 | pRUTH5-GST_SNRPD1-StrepII | E. coli expression of human SNRPD1 C-terminal domain with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II |
| 258261 | pXPR_050_MEP50-1 | Lentiviral expression of CRISPRi MEP50/WDR77 sgRNA #1 |
| 258262 | pXPR_050_MEP50-2 | Lentiviral expression of CRISPRi MEP50/WDR77 sgRNA #2 |
| 258263 | pLIX403_HA-SNRPD3_WT | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD3 |
| 258264 | pLIX403_HA-SNRPD3_RtoK | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD3 with arginine-to-lysine substitutions |
| 258265 | pLIX403_HA-SNRPD3_RtoA | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD3 with arginine-to-alanine substitutions |
| 258266 | pLIX403_HA-SNRPD1_WT | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD1 |
| 258267 | pLIX403_HA-SNRPD1_RtoK | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD1 with arginine-to-lysine substitutions |
| 258269 | pLEX307_PRMT5 | Lentiviral expression of C-terminal V5-tagged human PRMT5 |
| 258270 | pLEX304_MEP50 | Lentiviral expression of C-terminal V5-tagged human MEP50/WDR77 |
| 258273 | pDONR221_PRMT5 | Gateway entry plasmid for human PRMT5 |
| 258274 | pDONR221_MEP50 | Gateway entry plasmid for human MEP50/WDR77 |
| 258275 | pDONR221_HA-SNRPB_WT | Gateway entry plaSNRPid for N-terminal HA-tagged human SNRPB |
| 258276 | pDONR221_HA-SNRPB_RtoK | Gateway entry plasmid for N-terminal HA-tagged human SNRPB with arginine-to-lysine mutations |
| 258277 | pDONR221_HA-SNRPB_RtoA | Gateway entry plasmid for N-terminal HA-tagged human SNRPB with arginine-to-alanine mutations |
| 258278 | pDONR221_HA-SNRPD3_WT | Gateway entry plasmid for N-terminal HA-tagged human SNRPD3 |
| 258279 | pDONR221_HA-SNRPD3_RtoK | Gateway entry plasmid for N-terminal HA-tagged human SNRPD3 with arginine-to-lysine mutations |
| 258280 | pDONR221_HA-SNRPD3_RtoA | Gateway entry plasmid for N-terminal HA-tagged human SNRPD3 with arginine-to-alanine mutations |
| 258281 | pDONR221_HA-SNRPD1_WT | Gateway entry plasmid for N-terminal HA-tagged human SNRPD1 |
| 258282 | pDONR221_HA-SNRPD1_RtoK | Gateway entry plasmid for N-terminal HA-tagged human SNRPD1 with arginine-to-lysine mutations |
| 258283 | pDONR221_HA-SNRPD1_RtoA | Gateway entry plasmid for N-terminal HA-tagged human SNRPD1 with arginine-to-alanine mutations |
| 258284 | pDONR221_pICln | Gateway entry plasmid for human pICln/CLNS1A |
| 258285 | pDONR221_pICln_dPBM | Gateway entry plasmid for human pICln/CLNS1A lacking the C-terminal PBM |