Productive mRNA chromatin escape is promoted by PRMT5 activity.
DeAngelo JD, Maron MI, Roth JS, Hegde S, Silverstein AM, Gupta V, Stransky S, Basken J, Azofeifa J, Query CC, Sidoli S, Gamble MJ, Shechter D
Mol Cell. 2025 Nov 6;85(21):4016-4031.e9. doi: 10.1016/j.molcel.2025.09.021. Epub 2025 Oct 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 258246 | pXPR_050_PRMT5-3 | Lentiviral expression of CRISPRi PRMT5 sgRNA #3 |
| 258247 | pXPR_050_PRMT5-4 | Lentiviral expression of CRISPRi PRMT5 sgRNA #4 |
| 258248 | pXPR_050_CoPR5-1 | Lentiviral expression of CRISPRi CoPR5 sgRNA #1 |
| 258249 | pXPR_050_CoPR5-3 | Lentiviral expression of CRISPRi CoPR5 sgRNA #3 |
| 258250 | pXPR_050_pICln-2 | Lentiviral expression of CRISPRi pICln sgRNA #2 |
| 258251 | pXPR_050_pICln-3 | Lentiviral expression of CRISPRi pICln sgRNA #3 |
| 258252 | pXPR_050_RIOK1-1 | Lentiviral expression of CRISPRi RIOK1 sgRNA #1 |
| 258254 | pLIX403_HA-SNRPB_WT | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPB |
| 258261 | pXPR_050_MEP50-1 | Lentiviral expression of CRISPRi MEP50/WDR77 sgRNA #1 |
| 258262 | pXPR_050_MEP50-2 | Lentiviral expression of CRISPRi MEP50/WDR77 sgRNA #2 |
| 258263 | pLIX403_HA-SNRPD3_WT | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD3 |
| 258264 | pLIX403_HA-SNRPD3_RtoK | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD3 with arginine-to-lysine substitutions |
| 258265 | pLIX403_HA-SNRPD3_RtoA | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD3 with arginine-to-alanine substitutions |
| 258266 | pLIX403_HA-SNRPD1_WT | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD1 |
| 258267 | pLIX403_HA-SNRPD1_RtoK | Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD1 with arginine-to-lysine substitutions |
| 258269 | pLEX307_PRMT5 | Lentiviral expression of C-terminal V5-tagged human PRMT5 |