pDONR221_pICln_dPBM
(Plasmid
#258285)
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PurposeGateway entry plasmid for human pICln/CLNS1A lacking the C-terminal PBM
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
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Vector typeGateway Cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCLNS1A
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Alt namepICln
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SpeciesH. sapiens (human)
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Insert Size (bp)681
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MutationdPBM
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Entrez GeneCLNS1A (a.k.a. CLCI, CLNS1B, ICln)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221_pICln_dPBM was a gift from David Shechter (Addgene plasmid # 258285 ; http://n2t.net/addgene:258285 ; RRID:Addgene_258285) -
For your References section:
Productive mRNA chromatin escape is promoted by PRMT5 activity. DeAngelo JD, Maron MI, Roth JS, Hegde S, Silverstein AM, Gupta V, Stransky S, Basken J, Azofeifa J, Query CC, Sidoli S, Gamble MJ, Shechter D. Mol Cell. 2025 Nov 6;85(21):4016-4031.e9. doi: 10.1016/j.molcel.2025.09.021. Epub 2025 Oct 13. 10.1016/j.molcel.2025.09.021 PubMed 41086806