ssAAV-hSyn-hM3D(Gq)-eGFP
(Plasmid
#258326)
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PurposeDREADD construct expressing hM3D Gq fused with EGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258326 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonessAAV-hSyn-hM3D(Gq)-mCh2
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesSynthetic
- Promoter hSyn
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Tag
/ Fusion Protein
- hM3D (Gq) (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.05.08.723863 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ssAAV-hSyn-hM3D(Gq)-eGFP was a gift from Viviana Gradinaru (Addgene plasmid # 258326 ; http://n2t.net/addgene:258326 ; RRID:Addgene_258326) -
For your References section:
AAV tools enable functional modulation and readout of central and peripheral nervous systems in spiny mice. Chung JH, Donahue RR, Griffiths JA, Fan Y, Lin C, Chen X, Dutta S, Mazmanian S, Seifert AW, Gradinaru V. bioRxiv 2026.05.08.723863 10.64898/2026.05.08.723863