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pCMV-Flag-H3(1-28)-NrdJ-1C-H3.1(10-130)
(Plasmid #258599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258599 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCMV
  • Total vector size (bp) 4606
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flag-H3(1-28)-NrdJ-1C-H3.1(10-130)
  • Species
    H. sapiens (human); Marine Metagenome
  • Insert Size (bp)
    621
  • Mutation
    includes only aa 1-28 of H3 and aa 10-130 of H3.1
  • Promoter pCMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Flag-H3(1-28)-NrdJ-1C-H3.1(10-130) was a gift from Thomas Muir (Addgene plasmid # 258599 ; http://n2t.net/addgene:258599 ; RRID:Addgene_258599)

  • For your References section:

    Traceless protein semisynthesis in cells using the promiscuous ultra-fast split intein NrdJ-1. Ye X, Sokol J, Kofoed C, Dheer A, Zhang J, Muir TW. Chem Sci. 2026 Apr 28. doi: 10.1039/d6sc01808d. 10.1039/d6sc01808d PubMed 42058821
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