Traceless protein semisynthesis in cells using the promiscuous ultra-fast split intein NrdJ-1.
Ye X, Sokol J, Kofoed C, Dheer A, Zhang J, Muir TW
Chem Sci. 2026 Apr 28. doi: 10.1039/d6sc01808d.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 258592 | pET-His-SUMO-HA-MBP-NrdJ-1N | Expression of His-SUMO tagged HA-MBP-NrdJ-1 N intein in bacteria for protein splicing. NGTNPC as N-extein. |
| 258594 | pET-His-SUMO-NrdJ-1C(N145A)-SUMO | Expression of His-SUMO tagged NrdJ-1 C intein with an extra SUMO at C-ternimus in bacteria. Originally as splicing deficient mutant for ITC experiment |
| 258597 | pET-His-SUMO-NrdJ-1N (C76A) | Expression of His-SUMO tagged NrdJ-1 N intein in bacteria for protein semi-synthesis and splicing, with C76A mutation |
| 258598 | pET-His-SUMO-NrdJ-1C-H3.1(10-130) | Expression of NrdJ-1 C intein fused with histone H3.1(10-130) in bacteria |
| 258599 | pCMV-Flag-H3(1-28)-NrdJ-1C-H3.1(10-130) | Expression of NrdJ-1 C intein inserted in with histone H3.1 in mammalian cells. H3(1-28) is included to increase expression level |
| 258600 | pCMV-Flag-mCherry-progerin (NrdJ-1N) | Expression of NrdJ-1 N intein inserted into the C-terminal region of the progerin protein in mammalian cells |