Traceless protein semisynthesis in cells using the promiscuous ultra-fast split intein NrdJ-1.
Ye X, Sokol J, Kofoed C, Dheer A, Zhang J, Muir TW
Chem Sci. 2026 Apr 28. doi: 10.1039/d6sc01808d.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 258592 | pET-His-SUMO-HA-MBP-NrdJ-1N | Expression of His-SUMO tagged HA-MBP-NrdJ-1 N intein in bacteria for protein splicing. NGTNPC as N-extein. |
| 258597 | pET-His-SUMO-NrdJ-1N (C76A) | Expression of His-SUMO tagged NrdJ-1 N intein in bacteria for protein semi-synthesis and splicing, with C76A mutation |