pET-His-SUMO-HA-MBP-NrdJ-1N
(Plasmid
#258592)
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PurposeExpression of His-SUMO tagged HA-MBP-NrdJ-1 N intein in bacteria for protein splicing. NGTNPC as N-extein.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258592 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET
- Total vector size (bp) 7083
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHis-SUMO-HA-MBP-NrdJ-1N
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SpeciesMarine metagenome
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Insert Size (bp)1815
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Tags
/ Fusion Proteins
- 6xHis-SUMO (N terminal on backbone)
- HA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 reverse
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-His-SUMO-HA-MBP-NrdJ-1N was a gift from Thomas Muir (Addgene plasmid # 258592 ; http://n2t.net/addgene:258592 ; RRID:Addgene_258592) -
For your References section:
Traceless protein semisynthesis in cells using the promiscuous ultra-fast split intein NrdJ-1. Ye X, Sokol J, Kofoed C, Dheer A, Zhang J, Muir TW. Chem Sci. 2026 Apr 28. doi: 10.1039/d6sc01808d. 10.1039/d6sc01808d PubMed 42058821
Map uploaded by the depositor.
