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pGKTM1KN00431
(Plasmid #258606)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258606 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCk3 binary vector
  • Backbone manufacturer
    Dr. Nicola J. Patron
  • Backbone size w/o insert (bp) 6336
  • Total vector size (bp) 10912
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCmY-R2Tg[+2 A. thaliana introns]-tHSP
  • Species
    Taeniopygia guttata
  • Insert Size (bp)
    4529
  • Promoter pCmY
  • Tag / Fusion Protein
    • bipartite nuclear localization sequence (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.22.671877 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGKTM1KN00431 was a gift from Gözde Demirer (Addgene plasmid # 258606 ; http://n2t.net/addgene:258606 ; RRID:Addgene_258606)

  • For your References section:

    Optimized R2 retroelement complexes for DNA insertion into plant genomes. Muchenje KT, Wang Y, Oz TM, Li E, Saffron A, Demirer GS. Nat Biotech. 2026 Jun 19. 10.1038/s41587-026-03197-y
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