pGKTM2SP00619
(Plasmid #258607)

• Purpose: Level 2 intron-containing mCherry retrotransposition reporter flanked by wt R2Tg 5'/3' UTRs; TMV 5' UTR; R33/R4 rDNA homology arms; 3' HDV ribozyme; pCsB spectinomycin-resistant plant binary vector
• Depositing Lab: Gözde Demirer
• Depositing Organization: California Institute of Technology
  Located in United States of America
• Publication: Muchenje et al Nat Biotech. 2026 Jun 19.

Backbone

• Vector backbone: pCk2 binary vector
• Backbone manufacturer: Dr. Nicola J. Patron
• Backbone size w/o insert (bp): 6665
• Total vector size (bp): 9819
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pVmv-R33-wt R2Tg 5′ UTR-t35s-mCherry (A. thaliana intron)-TMV 5′ UTR-p35s-wt R2Tg 3′ UTR-R4-HDV-tHSP
• Species: Synthetic
• Insert Size (bp): 3108
• Promoter: pVmv
• Tag / Fusion Protein:
  • Bipartite nuclear localization sequence (N terminal on insert)

Cloning Information

• Cloning method: Golden Gate
• 5′ sequencing primer: Whole plasmid sequencing

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.22.671877 for bioRxiv preprint.