pHR_TRE3G_GFP-ligand
(Plasmid
#259143)
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PurposeInducible membrane expression of GFP fused to a PDGFRA transmembrane domain.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP ligand
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SpeciesSynthetic
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Insert Size (bp)933
- Promoter TRE-3G
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR_TRE3G_GFP-ligand was a gift from Leonardo Morsut (Addgene plasmid # 259143 ; http://n2t.net/addgene:259143 ; RRID:Addgene_259143) -
For your References section:
A novel reaction-diffusion architecture for engineering self-organized patterns in mammalian cells. Swedlund B, Danan JJ, Jiang TX, Ben Tahar S, Poon K, Bhamidipati PS, Kreiger ZA, Murillo S, Kunnan M, Pearce DJG, Chuong CM, Ehrenreich IM, Morsut L. bioRxiv [Preprint]. 2026 May 25:2026.05.24.727552. doi: 10.64898/2026.05.24.727552. 10.64898/2026.05.24.727552 PubMed 42244732