A novel reaction-diffusion architecture for engineering self-organized patterns in mammalian cells.

Swedlund B, Danan JJ, Jiang TX, Ben Tahar S, Poon K, Bhamidipati PS, Kreiger ZA, Murillo S, Kunnan M, Pearce DJG, Chuong CM, Ehrenreich IM, Morsut L
bioRxiv [Preprint]. 2026 May 25:2026.05.24.727552. doi: 10.64898/2026.05.24.727552. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
259143	pHR_TRE3G_GFP-ligand	Inducible membrane expression of GFP fused to a PDGFRA transmembrane domain.
259144	pHR_TRE3G_Gss-Myc-LaG16-LaG2_PGK_BFP	Inducible expression of a secreted anti-GFP nanobody dimer for inhibition of GFP-mediated synNotch signalling. A constitutive fluorescent reporter is linked for sorting infected cells.
259145	pHR_UAS_mCherry-PNE-ligand	Inducible membrane expression of mCherry fused to a PDGFRA transmembrane domain and a PNE peptide epitope.
259146	pHR_UAS_Gss-Myc-52SR4-LaM4_PGK_H2B-miRFP703	Inducible expression of a secreted anti-mCherry nanobody dimer for inhibition of mCherry-mediated synNotch signalling. A constitutive fluorescent reporter is linked for sorting infected cells.
259147	pHR_UAS_mCitrine-PEST	Inducible expression of cytoplasmic destabilized mCitrine.
259148	pHR_EF1a_Gss-NodalF1-Myc-LaG16-LaG2_PGK_BFP	Constitutive expression of a slow-diffusing secreted anti-GFP nanobody dimer fused to the Finger 1 domain of the mouse Nodal1 protein.
259149	pHR_TRE3G_Gss-NodalF1-Myc-LaG16-LaG2_PGK_BFP	Inducible expression of a secreted anti-GFP nanobody dimer fused to the Finger 1 domain of the mouse Nodal1 protein.
259150	pHR_SFFV_mCherry-T2A-Wnt3a	Constitutive expression of mouse Wnt3a concomitant to an intracellular fluorescent reporter, mCherry.
259151	pHR_TRE_Wnt3a_PGK_H2B-miRFP703	Inducible expression of endogenous mouse Wnt3a, with constitutive expression of a fluorescent reporter for cell sorting.
259152	pHR_UAS-TRE_Gss-Myc-LaG16-LaG2_PGK_BFP	Dual-input inducible expression of a secreted anti-GFP nanobody dimer for inhibition of GFP-mediated synNotch signalling. A constitutive fluorescent reporter is linked for sorting infected cells.
259153	pHR_TRE-UAS_Gss-55SR4-LaM4_PGK_H2B-miRFP703	Dual-input inducible expression of a secreted anti-mCherry nanobody dimer for inhibition of mCherry-mediated synNotch signalling. A constitutive fluorescent reporter is linked for sorting cells.