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pT1-B
(Plasmid #259183)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259183 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pVS1
  • Backbone manufacturer
    Sylvestre Marillonnet
  • Backbone size (bp) 4374
  • Modifications to backbone
    Extensive modification of the vector backbone to remove restriction sites, combined with complete redesign of the T-DNA border regions.
  • Vector type
    Plant Expression, Synthetic Biology
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.06.08.730999 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT1-B was a gift from Ahmad Khalil (Addgene plasmid # 259183 ; http://n2t.net/addgene:259183 ; RRID:Addgene_259183)

  • For your References section:

    Rational design of T-DNA vectors enables predictable, single-copy integration in Arabidopsis thaliana. Shaw WM, Gajendiran A, Tchantouridze EI, Bechen LL, Clarke SG, Guiziou S, Gehring M, Khalil AS. bioRxiv [Preprint]. 2026 Jun 10:2026.06.08.730999. doi: 10.64898/2026.06.08.730999. 10.64898/2026.06.08.730999 PubMed 42327332
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