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pET29b+_SpyCatcher_C5-15H
(Plasmid #259187)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259187 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET29b+
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 6557
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends BL21 Lemo21(DE3) E. coli for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    C5-15H
  • Species
    Synthetic
  • Insert Size (bp)
    1350
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • SpyCatcher (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b+_SpyCatcher_C5-15H was a gift from George Daley (Addgene plasmid # 259187 ; http://n2t.net/addgene:259187 ; RRID:Addgene_259187)

  • For your References section:

    Design of soluble Notch agonists that drive T cell development and boost immunity. Mout R, Jing R, Tanaka-Yano M, Egan ED, Eisenach H, Kononov MA, Windisch R, Najia MAT, Tompkins A, Hensch L, Bingham T, Gunage R, Zhao Y, Edman NI, Li C, Wang D, Schlaeger TM, Zon LI, North TE, Lendahl U, Rowe RG, Baker D, Blacklow SC, Daley GQ. Cell. 2025 Oct 16;188(21):5980-5994.e28. doi: 10.1016/j.cell.2025.07.009. Epub 2025 Aug 1. 10.1016/j.cell.2025.07.009 PubMed 40752493
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