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pBR227
(Plasmid #259191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259191 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PiggyBac
  • Backbone manufacturer
    Systems Biosciences
  • Backbone size w/o insert (bp) 4463
  • Total vector size (bp) 6317
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    iRFP713
  • Species
    Rhodopseudomonas palustris
  • Insert Size (bp)
    1077
  • Tags / Fusion Proteins
    • myristoylation sequence (N terminal on insert)
    • P2A sequence (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gcttcccgagctctataaaagagc
  • 3′ sequencing primer ccagaggttgattatcgataagc
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    FusionRed
  • Species
    Entacmaea quadricolor
  • Insert Size (bp)
    777
  • Mutation
    E176D mutation, introduced by removing BsaI cut sites
  • Tag / Fusion Protein
    • SV40 nuclear localization sequence (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.06.19.733436 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBR227 was a gift from Beatrice Ramm & Jared Toettcher (Addgene plasmid # 259191 ; http://n2t.net/addgene:259191 ; RRID:Addgene_259191)

  • For your References section:

    A modular live-cell biosensor for extracellular protease activity. Ramm B, Weatherly M, Toettcher J. bioRxiv 2026.06.19.733436 10.64898/2026.06.19.733436
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