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A modular live-cell biosensor for extracellular protease activity

Ramm B, Weatherly M, Toettcher J
bioRxiv 2026.06.19.733436 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
259190pBR181Constitutively expresses myristoylated iRFP and nuclear-localized TagBFP
259191pBR227Constitutively expresses myristoylated iRFP and nuclear-localized FusionRed
259192pBR254Constitutively expresses myristoylated FusionRed and nuclear-localized TagBFP
259193pBR167Constitutively expresses eNRGies biosensor with TEV-site and mEGFP (ECD) and FusionRed (ICD)
259194pBR222Constitutively expresses eNRGies biosensor with TEV-site and SEAP (ECD) and FusionRed (ICD)
259195pBR224Constitutively expresses eNRGies biosensor with TEV-site and SEAP (ECD) and Clover (ICD)
259196pBR225Constitutively expresses eNRGies biosensor with TEV-site and SEAP (ECD) and TagBFP (ICD)
259197pBR252Constitutively expresses eNRGies biosensor with TEV-site and SEAP (ECD) and HaloTag (ICD)
259198pBR253Constitutively expresses eNRGies biosensor with TEV-site and SEAP (ECD) and iRFP (ICD)
259199pBR188Constitutively expresses eNRGies biosensor with EK-site and mEGFP (ECD) and FusionRed (ICD)
259200pBR189Constitutively expresses eNRGies biosensor with FXa-site and mEGFP (ECD) and FusionRed (ICD)
259201pBR198Constitutively expresses eNRGies biosensor with MMP-9-site and mEGFP (ECD) and FusionRed (ICD)
259202pBR231Constitutively expresses control eNRGies biosensor with No-site and mEGFP (ECD) and FusionRed (ICD)
259203pBR235Constitutively expresses ADAM17 eNRGies biosensor with TGFa-site and mEGFP (ECD) and FusionRed (ICD)

Antibodies from Article