pBR253
(Plasmid #259198)

• Purpose: Constitutively expresses eNRGies biosensor with TEV-site and SEAP (ECD) and iRFP (ICD)
• Depositing Labs: Beatrice Ramm, Jared Toettcher
• Depositing Organization: Princeton University
  Located in the United States of America
• Publication: Ramm et al bioRxiv 2026.06.19.733436

Backbone

• Vector backbone: PiggyBac
• Backbone manufacturer: Systems Biosciences
• Backbone size w/o insert (bp): 4463
• Total vector size (bp): 7328
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: SEAP
• Alt name: ALPP
• Species: H. sapiens (human)
• Insert Size (bp): 1518
• Mutation: AA 6-511
• Tags / Fusion Proteins:
  • CD4 signal sequence (mouse) - G4S - linker (N terminal on insert)
  • 2x Myc tag (C terminal on insert)
  • TEV cleavage site (C terminal on insert)
  • 20 AA GS linker (C terminal on insert)
  • rat NRG TMD and juxtamembrane domain (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: iRFP
• Alt name: iRFP713
• Species: Rhodopseudomonas palustris
• Insert Size (bp): 948
• Tag / Fusion Protein:
  • SV40 nuclear localization sequence (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.64898/2026.06.19.733436 for bioRxiv preprint.