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pEF6_DmrB-MCS-V5-6xHis (KS248)
(Plasmid #259210)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259210 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pEF6
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site is SpeI, not destroyed in cloning. 3' cloning site is NotI, not destroyed in cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6_DmrB-MCS-V5-6xHis (KS248) was a gift from Kate Schroder (Addgene plasmid # 259210 ; http://n2t.net/addgene:259210 ; RRID:Addgene_259210)

  • For your References section:

    Caspase-4 dimerisation and D289 auto-processing elicit an interleukin-1beta-converting enzyme. Chan AH, Burgener SS, Vezyrgiannis K, Wang X, Acklam J, Von Pein JB, Pizzuto M, Labzin LI, Boucher D, Schroder K. Life Sci Alliance. 2023 Aug 9;6(10):e202301908. doi: 10.26508/lsa.202301908. Print 2023 Oct. 10.26508/lsa.202301908 PubMed 37558421
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