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Caspase-4 dimerisation and D289 auto-processing elicit an interleukin-1beta-converting enzyme.

Chan AH, Burgener SS, Vezyrgiannis K, Wang X, Acklam J, Von Pein JB, Pizzuto M, Labzin LI, Boucher D, Schroder K
Life Sci Alliance. 2023 Aug 9;6(10):e202301908. doi: 10.26508/lsa.202301908. Print 2023 Oct. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
259205pEF6_V5-DmrB-[delCARD]hCASP4-HA (KS697)Human caspase-4 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation, an N-terminal V5 tag and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259206pEF6_V5-DmrB-[delCARD]hCASP4(C258A)-HA (KS698)Catalytic mutant of human caspase-4 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation, an N-terminal V5 tag and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259207pEF6_V5-DmrB-[delCARD]hCASP4(D270A)-HA (KS707)D270A mutant of human caspase-4 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation, an N-terminal V5 tag and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259208pEF6_V5-DmrB-[delCARD]hCASP4(D289A)-HA (KS723)D289A mutant of human caspase-4 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation, an N-terminal V5 tag and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259209pEF6_V5-DmrB-[delCARD]hCASP4(D270A_D289A)-HA (KS728)D270A/D289A (IDL uncleavable) mutant of human caspase-4 with its CARD replaced by DmrB (FKBP12_F36V), an N-terminal V5 tag and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259210pEF6_DmrB-MCS-V5-6xHis (KS248)pEF6 empty vector for creating fusion proteins with N-terminal homodimerisation domain DmrB (FKBP12_F36V) for mammalian and bacterial expression.
259211pEF6_DmrB-[delCARD]hCASP4-HA (KS360)Human caspase-4 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259212pEF6_DmrB-[delCARD]hCASP4(C258A)-HA (KS361)Catalytic mutant (C258A) of human caspase-4 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259213pEF6_DmrB-[delCARD]hCASP4(D270A)-HA (KS740)D270A mutant of human caspase-4 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259214pEF6_DmrB-[delCARD]hCASP4(D289A)-HA (KS741)D89A mutant of human caspase-4 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259215pEF6_DmrB-[delCARD]hCASP1-HA (KS374)Human caspase-1 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259217pEF6_V5-pro_hIL1B (KS716)Canonical human pro-interleukin 1 beta (UniProt P01584) with an N-terminal V5 tag in pEF6 for mammalian and bacterial expression.
259218pEF6_V5-DmrB-hCASP4-HA (KS976)Canonical human full-length caspase-4 (UniProt P49662-1) with an N-terminal V5-DmrB (FKBP12_F36V) and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259219pEF6_V5-DmrB-hCASP4(C258A)-HA (KS725)Catalytic mutant (C258A) of human caspase-4 with N-terminal V5-DmrB (FKBP12_F36V) and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259220pEF6_DmrB-[delCARD]mCasp11 (KS650)Murine caspase-11 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation in pEF6 for mammalian and bacterial expression.
259221pEF6_V5-DmrB-[delCARD]hCASP1-HA (KS692)Human caspase-1 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation, an N-terminal V5 tag and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
259222pEF6_pro_mIL1b (KS52)Untagged canonical murine pro-interleukin 1beta (UniProt P10749) in pEF6 for mammalian and bacterial expression.

Antibodies from Article