pEF6_V5-DmrB-[delCARD]hCASP1-HA (KS692)
(Plasmid #259221)

• Purpose: Human caspase-1 with its CARD replaced by DmrB (FKBP12_F36V) for homodimerisation, an N-terminal V5 tag and a C-terminal HA tag in pEF6 for mammalian and bacterial expression.
• Depositing Lab: Kate Schroder
• Publication: Chan et al Life Sci Alliance. 2023 Aug 9;6(10):e202301908. doi: 10.26508/lsa.202301908. Print 2023 Oct.

Backbone

• Vector backbone: pEF6
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CASP1
• Alt name: caspase 1
• Alt name: ICE
• Species: H. sapiens (human)
• Entrez Gene: CASP1 (a.k.a. ICE, IL1BC, P45)
• Promoter: EF-1a, T7
• Tags / Fusion Proteins:
  • V5 (N terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 (TAATACGACTCACTATAGG)
• 3′ sequencing primer: BGH (TAGAAGGCACAGTCGAGG)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry