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pEF6_pro_mIL1b (KS52)
(Plasmid #259222)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259222 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pEF6
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Il1b
  • Alt name
    Il-1b
  • Alt name
    IL-1beta
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Il1b (a.k.a. IL-1beta, Il-1b)
  • Promoter EF-1a, T7

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer T7 (TAATACGACTCACTATAGG)
  • 3′ sequencing primer BGH (TAGAAGGCACAGTCGAGG)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6_pro_mIL1b (KS52) was a gift from Kate Schroder (Addgene plasmid # 259222 ; http://n2t.net/addgene:259222 ; RRID:Addgene_259222)

  • For your References section:

    Caspase-4 dimerisation and D289 auto-processing elicit an interleukin-1beta-converting enzyme. Chan AH, Burgener SS, Vezyrgiannis K, Wang X, Acklam J, Von Pein JB, Pizzuto M, Labzin LI, Boucher D, Schroder K. Life Sci Alliance. 2023 Aug 9;6(10):e202301908. doi: 10.26508/lsa.202301908. Print 2023 Oct. 10.26508/lsa.202301908 PubMed 37558421
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