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pCAGGS-DLL4(1-402)-SpyTag-6xHis
(Plasmid #259523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259523 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCAGGS
  • Total vector size (bp) 5799
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Delta-like ligand 4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1206
  • Mutation
    deleted amino acids 403-685
  • Entrez Gene
    DLL4 (a.k.a. AOS6, delta4, hdelta2)
  • Promoter CAG
  • Tag / Fusion Protein
    • SpyTag, 6xHis (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-DLL4(1-402)-SpyTag-6xHis was a gift from Stephen Blacklow (Addgene plasmid # 259523 ; http://n2t.net/addgene:259523 ; RRID:Addgene_259523)

  • For your References section:

    Design of soluble Notch agonists that drive T cell development and boost immunity. Mout R, Jing R, Tanaka-Yano M, Egan ED, Eisenach H, Kononov MA, Windisch R, Najia MAT, Tompkins A, Hensch L, Bingham T, Gunage R, Zhao Y, Edman NI, Li C, Wang D, Schlaeger TM, Zon LI, North TE, Lendahl U, Rowe RG, Baker D, Blacklow SC, Daley GQ. Cell. 2025 Oct 16;188(21):5980-5994.e28. doi: 10.1016/j.cell.2025.07.009. Epub 2025 Aug 1. 10.1016/j.cell.2025.07.009 PubMed 40752493
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