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PB-mPiezo1_Myc897_N2221K-IRES-TagRFP
(Plasmid #259536)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259536 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PiggyBac
  • Backbone manufacturer
    System Biosciences
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mPiezo1_Myc897_N2221K
  • Alt name
    Piezo1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    7674
  • Mutation
    N2221K
  • Entrez Gene
    PIEZO1 (a.k.a. DHS, ER, FAM38A, LMPH3, LMPHM6, Mib)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site AvrII (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ardem Patapoutian, Scripps Research

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-mPiezo1_Myc897_N2221K-IRES-TagRFP was a gift from Alexander Chesler (Addgene plasmid # 259536 ; http://n2t.net/addgene:259536 ; RRID:Addgene_259536)

  • For your References section:

    An inherited hypomorphic variant in PIEZO2 reveals structural features of mechanotransduction. Nickolls AR, Mulhall EM, Orlin DJ, Yarishkin O, Romero LO, Bell B, Hu X, Madden CA, Hardy RR, O'Brien GS, Donkervoort S, Saade DN, Lehky TJ, Foley AR, Bharucha-Goebel DX, Dastgir J, Vasquez V, Cordero-Morales JF, Bonnemann CG, Patapoutian A, Falgairolle M, Chesler AT. Neuron. 2026 Aug 24:S0896-6273(26)00585-4. doi: 10.1016/j.neuron.2026.07.030. 10.1016/j.neuron.2026.07.030 PubMed 42636812
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