An inherited hypomorphic variant in PIEZO2 reveals structural features of mechanotransduction.
Nickolls AR, Mulhall EM, Orlin DJ, Yarishkin O, Romero LO, Bell B, Hu X, Madden CA, Hardy RR, O'Brien GS, Donkervoort S, Saade DN, Lehky TJ, Foley AR, Bharucha-Goebel DX, Dastgir J, Vasquez V, Cordero-Morales JF, Bonnemann CG, Patapoutian A, Falgairolle M, Chesler AT
Neuron. 2026 Aug 24:S0896-6273(26)00585-4. doi: 10.1016/j.neuron.2026.07.030.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 259535 | PB-mPiezo1_Myc897-IRES-TagRFP | Expressing Myc-tagged mouse Piezo1 with a TagRFP fluorescent reporter. |
| 259536 | PB-mPiezo1_Myc897_N2221K-IRES-TagRFP | Expressing Myc-tagged N2221K-mutant mouse Piezo1 with a TagRFP fluorescent reporter. |
| 259537 | PB-TO-hPIEZO2_3xMyc1030-IRES-TagRFP | PiggyBac genomic insertion and dox-inducible expression of Myc-tagged human PIEZO2 and TagRFP. Requires separate expression of a tetracycline repressor (TetR). |
| 259539 | PB-TRE3Gbi-MCS-mScarlet3 | Empty all-in-one PiggyBac plasmid for genomic insertion and dox-inducible expression via a bi-directional TRE3G promoter. Includes an mScarlet3 reporter and an MCS for subcloning genes of interest. |
| 259540 | PB-TRE3Gbi-hPIEZO1-mScarlet3 | All-in-one PiggyBac plasmid for genomic insertion and dox-inducible expression of human PIEZO1 and mScarlet3. |
| 259541 | PB-TRE3Gbi-hPIEZO1_N2205K-mScarlet3 | All-in-one PiggyBac plasmid for genomic insertion and dox-inducible expression of N2205K-mutant human PIEZO1 and mScarlet3. |
| 259542 | PB-TRE3Gbi-hPIEZO2-mScarlet3 | All-in-one PiggyBac plasmid for genomic insertion and dox-inducible expression of human PIEZO2 and mScarlet3. |
| 259543 | PB-TRE3Gbi-hPIEZO2_N2434K-mScarlet3 | All-in-one PiggyBac plasmid for genomic insertion and dox-inducible expression of N2434K-mutant human PIEZO2 and mScarlet3. |
| 259544 | pcDNA3.1-mPiezo1-IRES-mScarlet3 | Expressing mouse Piezo1 with an mScarlet3 fluorescent reporter. |
| 259545 | pcDNA3.1-mPiezo1_N2221K-IRES-mScarlet3 | Expressing N2221K-mutant mouse Piezo1 with an mScarlet3 fluorescent reporter. |